![]() More specifically, this interaction produces two individual wave components with different velocities that fluctuate with the propagation direction through the specimen and that are each polarized in mutually perpendicular planes. It is then the interaction of plane-polarized light with a birefringent specimen that results in image contrast. ![]() In order for polarized light microscopes to reveal details that are poorly observed using traditional microscopy techniques, they must be equipped with a polarizer positioned in the light path before the specimen and an analyzer in the optical pathway between the observation tubes or camera port and the objective rear aperture. Polarized light microscopy is a contrast-enhancing technique that may be utilized for both quantitative and qualitative analysis of optically anisotropic specimens.
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